mraZ [2019-08-15 12:02:15]

Putative DNA-binding protein

locus: BSU_15130

pI: 4.97

mw: 16.43 kDa

protein length: 143 aa

gene length: 432 bp

function: unknown

product: unknown

essential: no

synonyms: yllB

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function]

Gene

Coordinates: 1,580,121 1,580,552

The protein

Protein family

• mraZ family (single member, according to UniProt)

[SW|Domains]

• 2 [SW|SpoVT-AbrB domain] (aa 7-47, aa 76-119) (according to UniProt)

Structure

• [PDB|1N0G] (the homolog from ''Mycoplasma pneumoniae'') [Pubmed|15146477]

[SW|Localization]

• nucleoid (heterogeneous) [Pubmed|16479537]

Expression and Regulation

Operons

• genes: [gene|A7131ACFA0CF5C222237C76E502D4AAD1F9BA3E4|mraZ]-[gene|1F894D6895CE13AA006CFE13A702E46C568F6E79|mraW]-[gene|79712F4CF884A660C71B45B3397AEFA5C0AFA683|ftsL]-[gene|EBEFF9E0A524DCDA19382E5401B923B805E4C559|pbpB]
  description: [Pubmed|8636036,8244929]

  regulation

  • constitutively expressed [Pubmed|15758244]

Biological materials

Mutant

• MGNA-B129 (yllB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1128 NBRP B. subtilis, Japan]
• 1A817 ( ''mraZ''::''erm''), [Pubmed|12682299], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A817&Search=1A817 BGSC]
• BKE15130 ([gene|A7131ACFA0CF5C222237C76E502D4AAD1F9BA3E4|mraZ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATCTCTTGCCCCACTT, downstream forward: _UP4_TAATGATTCTTCGTGTATAA
• BKK15130 ([gene|A7131ACFA0CF5C222237C76E502D4AAD1F9BA3E4|mraZ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATCTCTTGCCCCACTT, downstream forward: _UP4_TAATGATTCTTCGTGTATAA

References

• 8636036,16479537,8244929,16511046,15146477